Technology
Process
Step 1
Gene synthesis and vector construction
Step 2
DNA delivery into chloroplasts
Step 3
Selection and shoot regeneration
Step 4
Plant grown to maturity insoil and harvest seeds
Step 5
Large scale planting and harvest leaves
Step 6
Extract chloroplasts
Step 7
Lyse chloroplasts and purify protein
Step 8
Protein Characterisation (Quality Control)
Benefits
High Yielding
2-5 grams per kilogram of leaf material.
High biomass:
c.1MT of biomass/year for 10mX10m
Consistent
Gene silencing, position and epigenetic effects are absent in the chloroplast genetic system.
Therefore, there are unchanging levels of expression
Safe
Plant-based proteins expression has GRAS status and does not harbour harmful endotoxins or viral and prion contaminants, resulting in a simplified downstream process
Scalable
A highly scalable expression system for a continuous and sustainable production of recombinant proteins